• WIDAL SLIDE METHOD In Jodhpur

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Widal Test (Slide Agglutination) test helps to detect typhoid fever and paratyphoid fever ? collectively known as enteric fever, caused by the consumption of food or water contaminated by Salmonella bacteria. The symptoms include high fever, malaise, headache, constipation or diarrhoea, rose-colored spots on the chest, and enlarged spleen & liver. This test helps to detect antibodies in a blood sample against two antigens (O & H) of bacteria. It should only be performed after a week or two for accurate results. Please consult your health care practitioner to get an accurate diagnosis based on the symptoms, medical history and test results.
  • What is the Widal Slide Method?

    The Widal Slide Method is a diagnostic test used to detect antibodies in the blood against specific antigens of Salmonella bacteria that cause typhoid fever. It involves mixing patient serum with suspensions of different strains of Salmonella bacteria and observing for antibody-antigen reactions.

  • Why is the Widal Slide Method performed?

    The Widal Slide Method is performed to diagnose typhoid fever. It helps in detecting the presence of specific antibodies (O and H) in the patient's serum, indicating exposure or current infection with Salmonella typhi or Salmonella paratyphi, the bacteria responsible for typhoid fever.

  • What do the O and H antibodies represent in the Widal Slide Method?

    The O antibodies detect the presence of the "somatic" antigen on the surface of Salmonella bacteria, while the H antibodies detect the "flagellar" antigen. High levels of these antibodies indicate recent or active infection with typhoid bacteria.

  • How is the Widal Slide Method performed?

    In the Widal Slide Method, a patient's serum is mixed with a suspension of Salmonella bacteria on a specially coated glass slide. The slide is then observed for clumping or agglutination, which indicates a positive reaction and the presence of specific antibodies.

  • What results are considered positive in the Widal Slide Method?

    The interpretation of a positive reaction depends on the specific cut-off values and titers set by the laboratory performing the test. Usually, a rise in antibody titer (e.g., 1:160 or higher) is considered significant, indicating recent or current infection.

  • Can the Widal Slide Method give false-positive or false-negative results?

    Yes, the Widal Slide Method can yield false-positive or false-negative results. False positives can occur due to cross-reactivity with antibodies from other infections, while false negatives can result from various factors like testing too early in the infection or low antibody levels.

  • Does the Widal Slide Method diagnose other infections besides typhoid fever?

    The Widal Slide Method is specific for diagnosing typhoid fever caused by Salmonella typhi or Salmonella paratyphi. It does not diagnose other infections and should not be used as a general screening test.

  • Is the Widal Slide Method the only test for diagnosing typhoid fever?

    No, the Widal Slide Method is just one of the methods used to diagnose typhoid fever. Other tests, such as blood culture, polymerase chain reaction (PCR), and antibody detection assays like the Typhoid Rapid IgM/IgG Test, may also be used in conjunction with clinical and other laboratory findings.

  • Is the Widal Slide Method the only test for diagnosing typhoid fever?

    No, the Widal Slide Method is just one of the methods used to diagnose typhoid fever. Other tests, such as blood culture, polymerase chain reaction (PCR), and antibody detection assays like the Typhoid Rapid IgM/IgG Test, may also be used in conjunction with clinical and other laboratory findings.

  • How long does it take to get the results of the Widal Slide Method?

    The Widal Slide Method can provide preliminary results within a few hours, but the final interpretation may take longer, depending on the specific laboratory procedures and workload.

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